This state-of-the-art guide to gene editing technology features contributions from leading figures in the CRISPR-Cas field. Here you’ll find step-by-step protocols for applying CRISPR-Cas-based techniques in various biologic systems, including yeast, zebrafish, Drosophila, mice, and even human pluripotent stem cells! The contributors explain web-based tools and approaches for designing guide RNAs that precisely target genes of interest. They also describe and discuss methods for preparing and delivering CRISPR-Cas reagents into cells, and ways to screen for cells that harbor the desired genetic changes. Most important, the contributors describe strategies for optimizing CRISPR-Cas in each system, especially for minimizing off-target effects, and also discuss the development of next-generation CRISPR-Cas tools. In total, this book is an essential laboratory resource for all cell, molecular, and developmental biologists, as well as biochemists, geneticists, and others seeking to expand their biotechnology toolkits.